Glucosidase Inhibitory Activities of the Ethanolic Extract of Dioscorea Villosa Tubers
نویسنده
چکیده
Objective: The objective of this study was to evaluate αamylase inhibitory and α – glucosidase inhibitory activity of the ethanolic extract of Dioscorea villosa tubers in vitro. Materials and Methods: Different concentrations of the plant extract (1.5, 3, 7, 15.30, 60, 125, 250, 500 and 1000 μg/ml) were prepared in ethanol and subjected to α amylase inhibitory and α Glucosidase inhibitory assay. The absorbance was read at 540nm and 546nm respectively using spectrophotometer. Using this method, the percentage of α amylase inhibitory activity, α -glucosidase inhibitory activity and the IC50 values of each assay were calculated. Results: The ethanolic extract of Dioscorea villosa tubers exhibited appreciable αamylase inhibitory activity with an IC50 values 72.44μg/ml when compared with acarbose, IC50 value 83.23 ± 0.39 μg/mL and α glucosidase inhibitory activity with an IC50 value of 28.96 μg / ml when compared with acarbose IC50 value 35.03 ± 0.24 μg/mL Conclusion: This study supports the possible use of Dioscorea villosa tubers for diabetes because of its considerable α-amylase inhibitory activity as well as α glucosidase inhibitory activity.
منابع مشابه
Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism
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